Activin-A is up-regulated in severe asthma, attenuates allergic responses and is associated with angiogenesis

نویسندگان

  • Konstantinos Samitas
  • Sofia Tousa
  • Nikolaos Poulos
  • Eleftherios Zervas
  • Maria Semitekolou
  • Catherine M Hawrylowicz
  • Harsha Kariyawasam
  • Georgina Xanthou
  • Mina Gaga
چکیده

Methods Act-A expression was quantified in the serum, BALF (by ELISA) and bronchial tissue samples (IHC) obtained from CTRL (n=41), MMA (n=46) and SA (n=26). Act-A signaling expression (ActRIIA, ALK4, pSMad2/3) and remodeling markers (basement membrane thickness, goblet cell hyperplasia and angiogenesis) were also assessed in bronchial tissue (IHC/IF/Confocal). Moreover, naive T cells from atopic CTRLs and MMA/SA were isolated and cultured ex vivo alone or in the presence of allergen and/or rAct-A and/or dexamethasone and further utilized in co-cultures with naive T cells and in adoptive transfer experiments using NOD/ SCIDmice in a humanized model of experimental asthma.

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عنوان ژورنال:

دوره 3  شماره 

صفحات  -

تاریخ انتشار 2013